A mutation in the $\beta$ subunit of hemoglobin is discovered that reduces the affinity of 2,3-BPG binding. Which of the following mutations is most likely to have this consequence?
A) Val1 $\rightarrow$ Ile1
B) Asp99 $\rightarrow$ Glu99
C) Lys82 $\rightarrow$ Asp82
D) Glu6 $\rightarrow$ Val6

Question

Quizplus · Accepted Answer

Lysine and Aspartic acid have opposite charges, so substituting lysine with aspartic acid would change the electric nature of the binding site, which is known to reduce the binding affinity of 2,3-BPG. Other options involve substituting relatively neutral amino acids, which would have little effect on the binding.

A Mutation in The β\betaβ Subunit of Hemoglobin Is Discovered That Reduces the Affinity of That

A Mutation in The $\beta$ Subunit of Hemoglobin Is Discovered That Reduces the Affinity of That