In the second temperature phase of PCR, why does the temperature vary from 55 $\degree$C to 65 $\degree$C?
A) different ionic strengths
B) different primer concentrations
C) G-C content
D) different amount of hydrogen bonds

Question

Quizplus · Accepted Answer

The temperature varies during the second phase of PCR depending on the G-C content of the DNA template. Higher G-C content requires a higher annealing temperature, while lower G-C content requires a lower temperature. Therefore, the temperature range given in the question covers a range of G-C content that is usually found in DNA templates.

In the Second Temperature Phase of PCR, Why Does the Temperature