When a gene sequence is cloned using mRNA,the mRNA is isolated from the cell and converted into a double-stranded sequence using which enzyme?
A) DNA methylase
B) restriction endonucleases
C) reverse transcriptase
D) DNA ligase

Question

Quizplus · Accepted Answer

Reverse transcriptase is the enzyme used to convert mRNA into a double-stranded DNA sequence. This process is called reverse transcription and is often used in cloning genes from RNA viruses or in studying gene expression in eukaryotic cells. DNA methylase is an enzyme involved in DNA methylation, while restriction endonucleases and DNA ligase are enzymes used in recombinant DNA technology to cut and join DNA sequences.

When a Gene Sequence Is Cloned Using MRNA,the MRNA Is