In the second temperature phase of PCR,why does the temperature vary from 55 C to 65 C?
A) different ionic strengths
B) different primer concentrations
C) G-C content
D) different amount of hydrogen bonds

Question

In the second temperature phase of PCR,why does the temperature vary from 55  C to 65  C?
A) different ionic strengths 
B) different primer concentrations 
C) G-C content 
D) different amount of hydrogen bonds

Quizplus · Accepted Answer

The temperature range during the second phase of PCR (extension) varies from 55°C to 65°C because it is the optimal range for the Taq polymerase enzyme to elongate the DNA strand by adding nucleotides. The variation in temperature range is due to the difference in the GC content of the template DNA, as it affects the stability of the double helix. Higher GC content requires a higher temperature for denaturation, whereas lower GC content requires a lower temperature. Therefore, the extension temperature range is adjusted to accommodate different GC content of the template DNA.

In the Second Temperature Phase of PCR,why Does the Temperature