Which method of analyzing RNA transcripts is considered to be an unbiased approach because it does not use a predetermined collection of complementary DNA sequence?
A) plasmid cloning
B) viral transduction
C) RNA-seq
D) gene-expression microarrays

Question

Quizplus · Accepted Answer

RNA-seq is considered an unbiased approach because it doesn't rely on a predetermined collection of complementary DNA sequences. Instead, it uses high-throughput sequencing to sequence all the RNA transcripts in a sample, providing a comprehensive view of gene expression. Plasmid cloning and viral transduction can be useful for studying specific genes, but they are not unbiased approaches as they are designed to target specific sequences. Gene-expression microarray analysis relies on a predetermined set of probes, making it a biased approach.

Which Method of Analyzing RNA Transcripts Is Considered to Be