The maximum velocity (V_max) of an enzymatic reaction is an important piece of information regarding how the enzyme works.What series of measurements can be taken in order to infer the maximum velocity of an enzyme-catalyzed reaction?

Question

Quizplus · Accepted Answer

The best choice would be to measure the rate of substrate consumption shortly after mixing the enzyme and substrate for several substrate concentrations, since this will provide a range of values for the rate of the enzymatic reaction at different concentrations of the substrate. The other options may provide useful information, but they may not directly give us the maximum velocity of the reaction. Option A may provide an estimate of the equilibrium constant, but it does not necessarily provide the maximum velocity of the reaction. Option B may give a rate of product formation, but in some cases this may not be proportional to the rate of substrate consumption. Option C may give a rate of substrate consumption at high enzyme concentrations, but this may not provide information regarding the maximum velocity of the reaction.

The Maximum Velocity (Vmax) of an Enzymatic Reaction Is an Important

The Maximum Velocity (V_max) of an Enzymatic Reaction Is an Important