You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin. Figure 6-11
What part of the DNA replication process would be most directly affected if a strain of bacteria lacking DNA ligase were used to make the cell extracts?

Question

You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin.  Figure 6-11
What part of the DNA replication process would be most directly affected if a strain of bacteria lacking DNA ligase were used to make the cell extracts?

Quizplus · Accepted Answer

DNA ligase is responsible for completing the ligation, or joining, of Okazaki fragments on the lagging strand during DNA replication. Therefore, if a strain of bacteria lacking DNA ligase were used, lagging-strand completion would be most directly affected.

You Prepare Bacterial Cell Extracts by Lysing the Cells and Removing