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What Do You Predict Would Happen If You Created a TRNA

Question 26

Multiple Choice

What do you predict would happen if you created a tRNA with an anticodon of 5?-CAA-3? that is charged with methionine, and added this modified tRNA to a cell-free translation system that has all the normal components required for translating RNAs?  The Genetic Code \text { The Genetic Code }
 U  C  A  G  UUU Phe (F)   UCU Ser (S)   UAU Tyr (Y)   UGU Cys (C)   UUC -  UCC -  UAC -  UGC -  U  UUA Leu (L)   UCA –  UAA Stop  UGA Stop  UUG -  UCG -  UAG Stop  UGG Trp (W)   CUU Leu (L)   CCU Pro (P)   CAU His (H)   CGU Arg (R)   CUC -  CCC -  CAC -  CGC -  C CUA -  CCA -  CAA Gin (Q)   CGA -  CUG  CCG  CAG  CGG  AUU Ile (I)   ACU Thr (T)   AAU Asn (N)   AGU Ser (S)   AUC -  ACC -  AAC -  AGC -  A AUA -  ACA -  AAA Lys (K)   AGA Arg (R)   AUG Met (M)   ACG -  AAG -  AGG -  GUU Val (V)   GCU Ala (A)   GAU Asp (D)   GGU Gly (G)   GUC -  GCC -  GAC -  GGC -  G GUA -  GCA -  GAA Glu (E)   GGA -  GUG -  GCG -  GAG -  GGG - \begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array} Table 7-29


A) Methionine would be incorporated into proteins at some positions where glutamine should be.
B) Methionine would be incorporated into proteins at some positions where leucine should be.
C) Methionine would be incorporated into proteins at some positions where valine should be.
D) Translation would no longer be able to initiate.

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