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One Strand of a Section of DNA Isolated from the Bacterium

Question 47

Essay

One strand of a section of DNA isolated from the bacterium E.coli reads:
5′-GTAGCCTACCCATAGG-3′  The Genetic Code \text { The Genetic Code }
 U  C  A  G  UUU Phe (F)  UCU Ser (S)  UAU Tyr (Y)  UGU Cys (C)  UUC -  UCC -  UAC -  UGC -  U  UUA Leu (L)  UCA –  UAA Stop  UGA Stop  UUG -  UCG -  UAG Stop  UGG Trp (W)  CUU Leu (L)  CCU Pro (P)  CAU His (H)  CGU Arg (R)  CUC -  CCC -  CAC -  CGC -  C CUA -  CCA -  CAA Gin (Q)  CGA -  CUG  CCG  CAG  CGG  AUU Ile (I)  ACU Thr (T)  AAU Asn (N)  AGU Ser (S)  AUC -  ACC -  AAC -  AGC -  A AUA -  ACA -  AAA Lys (K)  AGA Arg (R)  AUG Met (M)  ACG -  AAG -  AGG -  GUU Val (V)  GCU Ala (A)  GAU Asp (D)  GGU Gly (G)  GUC -  GCC -  GAC -  GGC -  G GUA -  GCA -  GAA Glu (E)  GGA -  GUG -  GCG -  GAG -  GGG - \begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array}
Table 7-29
A.Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template.What will be the sequence of the mRNA in this region (make sure you label the 5′ and 3′ ends of the mRNA)?
B.How many different peptides could potentially be made from this sequence of RNA, assuming that translation initiates upstream of this sequence?
C.What are these peptides? (Give your answer using the one-letter amino acid code.)

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A.5′-GUAGCCUACCCAUAGG-3′
B.Two.(There ar...

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