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After Treating Cells with a Mutagen, You Isolate Two Mutants  The Genetic Code \text { The Genetic Code }

Question 52

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After treating cells with a mutagen, you isolate two mutants.One carries alanine and the other carries methionine at a site in the protein that normally contains valine.After treating these two mutants again with mutagen, you isolate mutants from each that now carry threonine at the site of the original valine (see Figure 7-67).Assuming that all mutations caused by the mutagen are due to single nucleotide changes, deduce the codons that are used for valine, alanine, methionine, and threonine at the affected site.  After treating cells with a mutagen, you isolate two mutants.One carries alanine and the other carries methionine at a site in the protein that normally contains valine.After treating these two mutants again with mutagen, you isolate mutants from each that now carry threonine at the site of the original valine (see Figure 7-67).Assuming that all mutations caused by the mutagen are due to single nucleotide changes, deduce the codons that are used for valine, alanine, methionine, and threonine at the affected site.    Figure 7-67  \text { The Genetic Code }   \begin{array}{|l|l|l|l|l|} \hline & \text { U } & \text { C } & \text { A } & \text { G } \\ \hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\ &\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\  \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\ &\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\ \hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\ &\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\  \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\ &\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\ \hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\ &\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\  \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\ &\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\ \hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\ &\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\  \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\ &\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\ \hline \end{array}   Table 7-29
Figure 7-67  The Genetic Code \text { The Genetic Code }
 U  C  A  G  UUU Phe (F)  UCU Ser (S)  UAU Tyr (Y)  UGU Cys (C)  UUC -  UCC -  UAC -  UGC -  U  UUA Leu (L)  UCA –  UAA Stop  UGA Stop  UUG -  UCG -  UAG Stop  UGG Trp (W)  CUU Leu (L)  CCU Pro (P)  CAU His (H)  CGU Arg (R)  CUC -  CCC -  CAC -  CGC -  C CUA -  CCA -  CAA Gin (Q)  CGA -  CUG  CCG  CAG  CGG  AUU Ile (I)  ACU Thr (T)  AAU Asn (N)  AGU Ser (S)  AUC -  ACC -  AAC -  AGC -  A AUA -  ACA -  AAA Lys (K)  AGA Arg (R)  AUG Met (M)  ACG -  AAG -  AGG -  GUU Val (V)  GCU Ala (A)  GAU Asp (D)  GGU Gly (G)  GUC -  GCC -  GAC -  GGC -  G GUA -  GCA -  GAA Glu (E)  GGA -  GUG -  GCG -  GAG -  GGG - \begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array}
Table 7-29

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