You are interested in understanding the gene regulation of Lkp1, a protein that is normally produced in liver and kidney cells in mice.Interestingly, you find that the LKP1 gene is not expressed in heart cells.You isolate the DNA upstream of the LKP1 gene, place it upstream of the gene for green fluorescent protein (GFP), and insert this entire piece of recombinant DNA into mice.You find GFP expressed in liver and kidney cells but not in heart cells, an expression pattern similar to the normal expression of the LKP1 gene.Further experiments demonstrate that there are three regions in the promoter, labeled A, B, and C in Figure 8-16, that contribute to this expression pattern.Assume that a single and unique transcription factor binds each site such that protein X binds site A, protein Y binds site B, and protein Z binds site C.You want to determine which region is responsible for tissue-specific expression, and create mutations in the promoter to determine the function of each of these regions.In Figure 8-16, if the site is missing, it is mutated such that it cannot bind its corresponding transcription factor. Figure 8-16
Which of the following proteins are likely to act as gene activators?

Question

You are interested in understanding the gene regulation of Lkp1, a protein that is normally produced in liver and kidney cells in mice.Interestingly, you find that the LKP1 gene is not expressed in heart cells.You isolate the DNA upstream of the LKP1 gene, place it upstream of the gene for green fluorescent protein (GFP), and insert this entire piece of recombinant DNA into mice.You find GFP expressed in liver and kidney cells but not in heart cells, an expression pattern similar to the normal expression of the LKP1 gene.Further experiments demonstrate that there are three regions in the promoter, labeled A, B, and C in Figure 8-16, that contribute to this expression pattern.Assume that a single and unique transcription factor binds each site such that protein X binds site A, protein Y binds site B, and protein Z binds site C.You want to determine which region is responsible for tissue-specific expression, and create mutations in the promoter to determine the function of each of these regions.In Figure 8-16, if the site is missing, it is mutated such that it cannot bind its corresponding transcription factor.  Figure 8-16
Which of the following proteins are likely to act as gene activators?

Quizplus · Accepted Answer

The experiment showed that the promoter regions A, B, and C contribute to tissue-specific expression of the LKP1 gene. It was also shown that a single and unique transcription factor binds each site, with protein X binding site A, protein Y binding site B, and protein Z binding site C. Since the goal is to determine which region is responsible for tissue-specific expression, it is important to consider which transcription factors are likely to act as gene activators. Gene activators are transcription factors that promote gene expression by binding to the promoter region and recruiting RNA polymerase to initiate transcription. Factors X and Y are likely to be gene activators because their binding to regions A and B, respectively, is associated with expression in liver and kidney cells. Therefore, the answer is A) factors X and Y.

You Are Interested in Understanding the Gene Regulation of Lkp1