You Are Interested in the Regulation of Gene Q $\text { experiment }$

You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site "A" but cannot bind to site "A" at the same time.Protein J binds to site "B" on the promoter.The promoter region is diagrammed in Figure 8-20. Figure 8-20
You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20. $\text { experiment }$$\text { reaulator protein added }$$\text { Q mRNA }$
$\begin{array}{ccccc}\text { number } &\quad\quad\quad\text { G } & H & J & \quad\quad\text { made? } \\\hline 1 &\quad\quad - & - & - & \text { no } \\\hline 2 & \quad\quad+ & - & - & \text { no } \\\hline 3 & \quad\quad- & + & - & \text { yes } \\\hline 4 &\quad\quad - & - & + & \text { yes } \\\hline 5 &\quad\quad + & + & - & \text { no } \\\hline 6 &\quad\quad + & - & + & \text { yes } \\\hline 7 & \quad\quad- & + & + & \text { yes } \\\hline 8 & \quad\quad+ & + & + & \text { yes }\end{array}$


Table 8-20
Your colleague looks at your data above and predicts that protein G will bind more strongly to the DNA at site A, compared to protein H.Which experiment above is critical for this prediction?

A) #2
B) #3
C) #5
D) #6