A DNA library has been constructed by purifying chromosomal DNA from mice, cutting the DNA with the restriction enzyme NotI, and inserting the fragments into the NotI site of a plasmid vector.What information CANNOT be retrieved from this library?

Question

Quizplus · Accepted Answer

DThe DNA library constructed from chromosomal DNA and a plasmid vector can provide information on gene regulatory sequences (A) and intron sequences (B) since these are part of the chromosomal DNA. However, telomere sequences (C) are typically not present in such libraries because they are repetitive, difficult to clone, and often not cut by restriction enzymes like NotI. Amino acid sequences of proteins (D) cannot be directly retrieved from a DNA library because the library contains DNA sequences, not proteins; amino acid sequences are inferred from the DNA sequence through transcription and translation processes, which are not part of the library itself.

A DNA Library Has Been Constructed by Purifying Chromosomal DNA