With fully automated Sanger sequencing, all four chain-terminating ddNTPs are added into a single reaction and loaded onto a capillary gel.The sequence can be determined even though all ddNTPs are mixed together because

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The Sanger sequencing method generates a series of DNA fragments that terminate at each base along the sequence being analyzed. Each terminating ddNTP is labeled with a different fluorescent dye, allowing the sequence to be determined by detecting the color of the fluorescence at each position as the fragments pass through the capillary gel. This color indicates the identity of the terminating base at the end of each fragment.

With Fully Automated Sanger Sequencing, All Four Chain-Terminating DdNTPs Are