Figure 10-45A depicts the restriction map of one segment of the human genome for four restriction nucleases W, X, Y, and Z.Figure 10-45B depicts the restriction maps of four individual BAC clones that contain segments of human DNA from the region depicted in Figure 10-45A. (A)

(B)

Figure 10-45
From this information, how would you order these BAC clones, from left to right?

Question

Figure 10-45A depicts the restriction map of one segment of the human genome for four restriction nucleases W, X, Y, and Z.Figure 10-45B depicts the restriction maps of four individual BAC clones that contain segments of human DNA from the region depicted in Figure 10-45A. (A)

(B)

Figure 10-45
From this information, how would you order these BAC clones, from left to right?

(B)

Figure 10-45
From this information, how would you order these BAC clones, from left to right?

Quizplus · Accepted Answer

To order the BAC clones from left to right, we need to compare the restriction maps of each clone with the known restriction map of the human genome segment. 
- BAC 2 has a restriction map that matches perfectly with the left side of the human genome segment.
- BAC 1 has a restriction map that matches the left side of the human genome segment, but with an extra restriction site for enzyme Y in the middle.
- BAC 4 has a restriction map that matches the right side of the human genome segment, but with an extra restriction site for enzyme X in the middle.
- BAC 3 has a restriction map that matches the right side of the human genome segment, but with an extra restriction site for enzyme W in the middle.

Therefore, the correct order from left to right is BAC 2, BAC 1, BAC 4, BAC 3, which corresponds to answer choice B.

Figure 10-45A Depicts the Restriction Map of One Segment of the Human