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You Have the Amino Acid Sequence of a Protein and Wish

Question 53

Essay

You have the amino acid sequence of a protein and wish to design PCR primers that will allow you to amplify this gene by PCR from genomic DNA.Using this protein sequence, you deduce a particular DNA sequence that can encode this protein.Why is it unwise to use only this DNA sequence you have deduced to design PCR primers for isolating the gene encoding your protein of interest from genomic DNA?

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Because most amino acids can be encoded ...

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