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You Are Studying a Protein That Contains the Peptide Sequence  The Genetic Code \text { The Genetic Code }

Question 55

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You are studying a protein that contains the peptide sequence RDWKLVI.The part of the DNA encoding this peptide is included in the sequence shown below.  The Genetic Code \text { The Genetic Code }
 U  C  A  G  UUU Phe (F)  UCU Ser (S)  UAU Tyr (Y)  UGU Cys (C)  UUC -  UCC -  UAC -  UGC -  U  UUA Leu (L)  UCA –  UAA Stop  UGA Stop  UUG -  UCG -  UAG Stop  UGG Trp (W)  CUU Leu (L)  CCU Pro (P)  CAU His (H)  CGU Arg (R)  CUC -  CCC -  CAC -  CGC -  C CUA -  CCA -  CAA Gin (Q)  CGA -  CUG  CCG  CAG  CGG  AUU Ile (I)  ACU Thr (T)  AAU Asn (N)  AGU Ser (S)  AUC -  ACC -  AAC -  AGC -  A AUA -  ACA -  AAA Lys (K)  AGA Arg (R)  AUG Met (M)  ACG -  AAG -  AGG -  GUU Val (V)  GCU Ala (A)  GAU Asp (D)  GGU Gly (G)  GUC -  GCC -  GAC -  GGC -  G GUA -  GCA -  GAA Glu (E)  GGA -  GUG -  GCG -  GAG -  GGG - \begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array}
Table 10-56
5′-GGCGTGACTGGAAGCTAGTCATC-3′
3′-CCGCACTGACCTTCGATCAGTAG-5′
This sequence does not contain any HindIII restriction enzyme sites; the target sequence for the HindIII restriction nuclease is shown in Figure 10-65.  You are studying a protein that contains the peptide sequence RDWKLVI.The part of the DNA encoding this peptide is included in the sequence shown below.  \text { The Genetic Code }   \begin{array}{|l|l|l|l|l|} \hline & \text { U } & \text { C } & \text { A } & \text { G } \\ \hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\ &\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\  \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\ &\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\ \hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\ &\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\  \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\ &\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\ \hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\ &\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\  \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\ &\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\ \hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\ &\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\  \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\ &\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\ \hline \end{array}   Table 10-56 5′-GGCGTGACTGGAAGCTAGTCATC-3′ 3′-CCGCACTGACCTTCGATCAGTAG-5′ This sequence does not contain any HindIII restriction enzyme sites; the target sequence for the HindIII restriction nuclease is shown in Figure 10-65.    Figure 10-65A Your goal is to create a HindIII site on this plasmid without changing the coding sequence of the protein.Explain how you would do this.
Figure 10-65A
Your goal is to create a HindIII site on this plasmid without changing the coding sequence of the protein.Explain how you would do this.

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