You are studying a protein that contains the peptide sequence RDWKLVI.The part of the DNA encoding this peptide is included in the sequence shown below.
Table 10-56
5′-GGCGTGACTGGAAGCTAGTCATC-3′
3′-CCGCACTGACCTTCGATCAGTAG-5′
This sequence does not contain any HindIII restriction enzyme sites; the target sequence for the HindIII restriction nuclease is shown in Figure 10-65.
Figure 10-65A
Your goal is to create a HindIII site on this plasmid without changing the coding sequence of the protein.Explain how you would do this.
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