Which of the following molecular techniques involves "DNA replication in a tube"?

Question

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PCR (polymerase chain reaction) involves DNA replication in a tube, by using specific primers and DNA polymerase to amplify a specific target region of DNA. Transformation is a process by which DNA is introduced into a cell, gel electrophoresis is a technique used to separate fragments of DNA based on their size, and RFLP (restriction fragment length polymorphism) is a technique used to analyze differences in DNA sequences by cutting them with specific enzymes.

Which of the Following Molecular Techniques Involves "DNA Replication in a Tube