In order to join a fragment of human DNA to bacterial or yeast DNA, both the human DNA and the bacterial or yeast DNA must first be treated with the same:

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Restriction enzymes are necessary for cutting the DNA at specific sites, allowing for the insertion of the fragment from another source. Both the human DNA and bacterial or yeast DNA need to be cut by the same restriction enzyme in order for them to have compatible ends for ligating together. DNA ligase is then used to join the fragments together. DNA polymerase is involved in DNA replication and repair, not in joining fragments from different sources. DNA gyrase is a bacterial enzyme involved in DNA supercoiling and not relevant to this process.

In Order to Join a Fragment of Human DNA to Bacterial