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You Have Constructed a Genomic Library of Felis Catus (Domestic

Question 9

Multiple Choice

You have constructed a genomic library of Felis catus (domestic cat) in E.coli.You extract the plasmid DNA from the clones,digest the plasmid with restriction enzymes,separate the resulting fragments by gel electrophoresis,and transfer the fragments to a nylon membrane by the Southern blot procedure.You then use a fluorescently labeled probe that is complementary to a gene of interest that may be involved in feline lymphoma.The DNA corresponding to one clone yields a single fluorescent DNA band after probing the Southern blot.Based on this information


A) all of the clones tested,except the one described,have the gene of interest.
B) only the one clone described has the gene of interest.
C) the gene of interest is located entirely on the fluorescent DNA band.
D) the part of the gene complementary to the probe is located on the fluorescent DNA band.
E) only the one clone described contains the part of the gene complementary to the probe on the fluorescent DNA band.

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