Gene knockdown via RNAi relies on the ability of eukaryotic cells to
A)degrade target mRNAs that are complementary to introduced dsRNA.
B)induce microRNA expression under particular conditions.
C)preferentially degrade mutated mRNAs.
D)inhibit transcription of sets of genes based on the presence of distal control elements.
E)preferentially degrade mutated mRNAs by inducing microRNA expression under particular conditions.

Question

Quizplus · Accepted Answer

Gene knockdown via RNA interference (RNAi) operates by introducing double-stranded RNA (dsRNA) into a cell, which is then processed into small interfering RNAs (siRNAs). These siRNAs guide the RNA-induced silencing complex (RISC) to target mRNAs that are complementary to the introduced dsRNA, leading to their degradation. This process does not directly involve the induction of microRNA expression, the preferential degradation of mutated mRNAs, or the inhibition of transcription based on distal control elements.

Gene Knockdown Via RNAi Relies on the Ability of Eukaryotic