The recognition site for a restriction enzyme is 5′ ACCGG^T 3′.The ^ symbol indicates the site of cleavage.After digestion of DNA with this restriction enzyme, how will the ends of the resulting DNA fragments look?
A)They will have sticky, single-stranded ends with 5′ overhangs.
B)They will have sticky, single-stranded ends with 3′ overhangs.
C)They will have blunt ends.
D)They will have sticky, double-stranded ends.
E)The result cannot be predicted using only the information given.

Question

Quizplus · Accepted Answer

The cleavage site indicated by the ^ symbol in 5′ ACCGG^T 3′ suggests that after digestion, the DNA will be cut in such a way to leave overhangs. Since the cut is made after the G, and given the directionality (5′ to 3′), the overhangs will be on the 5′ end, resulting in sticky, single-stranded ends with 5′ overhangs.

The Recognition Site for a Restriction Enzyme Is 5′ ACCGG^T