Is There Any Method by Which We Can Detect Individual

Is there any method by which we can detect individual atoms in the laboratory?

A) Yes, by using microscopes based on X-rays rather than on light.
B) Yes, by using very high power microscopes based on visible light of very short wavelength.
C) No, because atoms are purely mathematical abstractions and not real physical objects.
D) No, because atoms are smaller than any physical wavelengths.
E) Yes, by using microscopes based on the "matter waves" made by material particles such as electrons.