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A Recipient Gal- Bio- Attl- Strain of E gal+bioattl71\quad \mathrm { gal } ^ { + } \mathrm { bio } ^ { - } \mathrm { attl } ^ { - } \quad 71

Question 45

Multiple Choice

A recipient gal- bio- attl- strain of E. coli is transduced by P1 phage from a donor wild-type gal+ bio+ attl+ strain. (attl- is a mutant which can't form a l prophage because the site for l integration is missing or defective.) gal+ transductants are selected for by plating the recipient cells on a Moderate with galactose as the sole carbon or energy source. Replica plating and testing for lysogenic ability give the complete genotypes of 106 transductants: Class 1: gal+bioattl71\quad \mathrm { gal } ^ { + } \mathrm { bio } ^ { - } \mathrm { attl } ^ { - } \quad 71
Class 2: gal+bio+attl\quad \mathrm { gal } ^ { + } \mathrm { bio } ^ { + }attl^- 0\quad 0
Class 3: gal+bio\quad\mathrm { gal } ^ { + }bio^- attl+9a t t l ^ { + } \quad 9
Class 4: gal+bio+attl+26\quad \mathrm { gal } ^ { + } \mathrm { bio } ^ { + } \mathrm { attl } ^ { + } \quad 26 What is the frequency of cotransduction between gal and attl?


A) 33%
B) 25%
C) 8.5%
D) 67%
E) 46%

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