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In E Coli, a P1 Generalized Transduction Was Carried Out Using

Question 42

Multiple Choice

In E. coli, a P1 generalized transduction was carried out using a donor strain of ara+ leu+ ilvH+ genotype. The recipient strain was ara- leu- ilvH-. 381 ara+ transductants were first recovered and then the remaining genotypes of these 381 were determined and the results are shown below: ara+leuilvH=32ara+leu+ilvH=9ara+leuilvH+=0ara+leu+llvH+=340\begin{array} { l } \mathrm { ara } ^ { + } \text {leu}^-{ ilvH }^- \quad = 32 \\\mathrm { ara } ^ { + } \mathrm { leu } ^ { + } i \mathrm { lv } \mathrm { H } ^ { - } = 9 \\\mathrm { ara } ^ { + } \operatorname { leu }^- i \mathrm { lv } \mathrm { H } ^ { + } = 0 \\\mathrm { ara } ^ { + } \mathrm { leu } ^ { + } \mathrm { llv } \mathrm { H } ^ { + } \quad = 340 \\\end{array} Which gene is in the middle?


A) ara
B) leu
C) ilvH
D) ara or ilvH
E) leu or ara

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