A section of a genome is cut with three enzymes: A, B, and C. Cutting with A and B yields a 10-kb fragment. Cutting with B and C yields a 2-kb fragment. What is the expected result from a digest with A and C, if the C site lies in between the A and B sites?
A) a 12-kb fragment
B) an 8-kb fragment
C) an 8-kb and a 2-kb fragment
D) a 10-kb and a 2-kb fragment
E) a 10-kb, an 8-kb, and a 2-kb fragment

Question

Quizplus · Accepted Answer

The C site is between the A and B sites, so cutting with A and C will remove the 2-kb fragment, leaving an 8-kb fragment.

A Section of a Genome Is Cut with Three Enzymes