You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain.
2) Digest the gold gene and a Ti plasmid with appropriate restriction enzymes.
3) Insert the gold gene into the Ti plasmid.
4) Amplify the gold gene with PCR.
5) Transfer the recombinant Ti plasmid into Agrobacterium tumefaciens.
6) Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome.

Question

Quizplus · Accepted Answer

First, amplify the gold gene with PCR (4), then digest both the gene and the Ti plasmid with restriction enzymes (2), insert the gene into the plasmid (3), transfer the recombinant plasmid into Agrobacterium (5), infect the plant with the modified Agrobacterium (1), and finally, use a selectable marker to identify successful integrations (6).

You Have Discovered a Gene That Enables Organisms to Accumulate