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A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After subjecting his PCR reaction to gel electrophoresis, the student sees no PCR product on the gel. What error(s) might he have made? (Select all that apply.)

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Use the following to answer questions 
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After subjecting his PCR reaction to gel electrophoresis, the student sees no PCR product on the gel. What error(s) might he have made? (Select all that apply.)

Quizplus · Accepted Answer

Step 2 at too high a temperature could prevent primer annealing, and step 3 for too short a time might not allow sufficient DNA extension.

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