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A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After electrophoresing his PCR reaction, the student sees the desired PCR product on the gel as well as several smaller bands. What error(s) might he have made? (Select all that apply.)

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Use the following to answer questions 
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After electrophoresing his PCR reaction, the student sees the desired PCR product on the gel as well as several smaller bands. What error(s) might he have made? (Select all that apply.)

Quizplus · Accepted Answer

Step 2 at too low a temperature can lead to non-specific binding, repetitive sequences in primers can cause mispriming, and contamination can introduce additional DNA templates, all resulting in extra bands.

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