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You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA. Southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice. The two mice strains are crossed to produce an F₁ generation. Two F₁ siblings are mated to produce a dozen F₂ progeny. You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe.
-How many bands would be seen in the Southern blot of an F₁ individual?

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An F1 individual would be heterozygous, inheriting one allele from each parent, resulting in two different bands (2-kb and 4-kb) on the Southern blot.

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