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A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site. After dideoxy sequencing, the gel pattern shown in this diagram is obtained.
-On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was closest to the primer?

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Use the following to answer questions 
A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site. After dideoxy sequencing, the gel pattern shown in this diagram is obtained. 
-On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was closest to the primer?

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A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site. After dideoxy sequencing, the gel pattern shown in this diagram is obtained. 
-On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was closest to the primer?

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