A Gene in the Fruit Fly D

A gene in the fruit fly D. melanogaster is found to affect neuronal function when mutated. A homologue of this gene is identified in mice. Which of the following methods would it make most sense for a scientist to use to study the function of the gene in mice? (Select all that apply.)

A) Forward genetics
B) Reverse genetics
C) Mutagenesis with radiation
D) CRISPR-Cas9 genome editing targeting the gene of interest followed by nonhomologous end joining (NHEJ)
E) CRISPR-Cas9 genome editing targeting the gene of interest followed by homologous recombination (HR) with a donor template