An E. coli strain of chromosomal genotype lacI^- lacP⁺lacO⁺ lacZ⁺ lacY⁺ constitutively expresses the genes of the lac operon. The strain is converted to wild-type lac operon regulation by the addition of an extra piece of DNA. What gene or genes are contained on this extra DNA that explain this conversion to wild type? Include an explanation of cis- or trans-acting factors and how they work.

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The Answer of An E. coli strain of chromosomal genotype lacI^- lacP⁺lacO⁺ lacZ⁺ lacY⁺ constitutively expresses the genes of the lac operon. The strain is converted to wild-type lac operon regulation by the addition of an extra piece of DNA. What gene or genes are contained on this extra DNA that explain this conversion to wild type? Include an explanation of cis- or trans-acting factors and how they work.

An E Coli Strain of Chromosomal Genotype LacI- LacP+ lacO+ LacZ+

An E Coli Strain of Chromosomal Genotype LacI^- LacP⁺lacO⁺ LacZ⁺