The DNA double helix can be separated into single strands using
A) NaOH.
B) heat.
C) reverse transcriptase.
D) either heat or NaOH.
E) heat, NaOH, and reverse transcriptase.

Question

Quizplus · Accepted Answer

Both heat and NaOH can denature DNA, separating the double helix into single strands. Heat disrupts hydrogen bonds, while NaOH raises the pH, leading to strand separation. Reverse transcriptase does not separate DNA strands; it synthesizes DNA from an RNA template.

The DNA Double Helix Can Be Separated into Single Strands