Using SYBR green as a detection dye in real time PCR is more specific than traditional PCR, but it is not as sensitive.

Question

Quizplus · Accepted Answer

SYBR Green binds to double-stranded DNA and is used in real-time PCR to monitor the amplification process, but it does not affect the specificity or sensitivity of the PCR itself compared to traditional PCR. The specificity and sensitivity are more influenced by the primer design and PCR conditions.

Using SYBR Green as a Detection Dye in Real Time