Question 1

Explain how reverse transcriptase PCR can be used to accurately quantify RNA in different samples.

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Question 2

Describe the principle of TaqMan real- time PCR.

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Question 3

List ways in which contamination of PCR reactions can be minimized.

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Question 4

Discuss the factors that must be considered when designing primers for a PCR reaction.

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Question 5

How can one quantify the amount of nucleic acid that is present in a sample and determine its purity?

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Quiz 13: Amplification Techniques

Explain how reverse transcriptase PCR can be used to accurately quantify RNA in different samples.