Which of the following is the correct sequence of PCR?
A) Heat to 70°C (to separate DNA) , then cool to 50°C (to bind primers and enzymes) , and then reheat to 90°C (to synthesize DNA) .
B) Heat to 90°C (to separate DNA) , then cool to 50°C (to bind primers and enzymes) , and then reheat to 70°C (to synthesize DNA) .
C) Heat to 70°C (to bind primers and enzymes) , then cool to 50°C (to synthesize DNA) , and then reheat to 90°C (to separate DNA) .
D) Heat to 90°C (to bind primers and enzymes) , then cool to 50°C (to separate DNA) , and then reheat to 70°C (to synthesize DNA) .
Correct Answer:
Verified
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