Question 1

In what way was the interrupted mating technique used to generate a genetic map in
E. coli based on time?

Accepted Answer

The interrupted mating technique showed that genes were passed in a linear
fashion from the Hfr bacterial strain to an F - strain. By interrupting the mating tube, it could be determined when (in minutes) genes were transferred.

Question 2

Bacteriophages engage in two interactive cycles with bacteria. What are these cycles?

Accepted Answer

Bacteriophages can undergo the lytic cycle, where they replicate within and lyse the host cell, and the lysogenic cycle, where they integrate their DNA into the host genome and replicate along with it without causing immediate harm.

Question 3

Name two forms of recombination in bacteria.

Accepted Answer

Conjugation and transduction are two forms of genetic recombination in bacteria, where conjugation involves direct transfer of DNA between bacteria, and transduction involves transfer of DNA by a virus.

Question 4

Jacob, Wollman, and others developed a linkage map of E. coli that is based on time. What form of recombination is involved in generating a linkage map based on time?

Accepted Answer

The answer of Jacob, Wollman, and others developed a linkage...

Question 5

Name the typical phases of the bacterial growth cycle in liquid culture medium.

Accepted Answer

The answer of Name the typical phases of the bacterial...

Question 6

How does an auxotroph differ from a prototroph?

Accepted Answer

The answer of How does an auxotroph differ from a...

Question 7

In general, what two methods are used to grow bacteria in the laboratory?

Accepted Answer

The answer of In general, what two methods are used...

Question 8

Describe how different strains of E. coli can reveal different linkage arrangements of genes in Hfr crosses.

Accepted Answer

The answer of Describe how different strains of E. coli...

Question 9

Transduction is a form of recombination in bacteria that involves

Accepted Answer

Transduction is a process of genetic recombination in bacteria where bacteriophages (viruses that infect bacteria) transfer genetic material from one bacterium to another.

Question 10

Distinguish between F+ and F - bacteria.

Accepted Answer

The answer of Distinguish between F+ and F - bacteria....

Question 11

Name the general category into which double -stranded circular extrachromosomal DNA elements such as F factors, ColE1, and R would fall.

Accepted Answer

Plasmids are double-stranded circular extrachromosomal DNA elements found in bacteria, which include F factors, ColE1, and R factors.

Question 12

Assume that the gene trpA in an auxotrophic strain of E. coli is located at 27 minutes, whereas the gene pyrE is located at 81 minutes. How are minutes arrived at in the
context of gene mapping in bacteria?

Accepted Answer

The answer of Assume that the gene trpA in an...

Question 13

What is a merozygote?

Accepted Answer

The answer of What is a merozygote?...

Question 14

Explain the composition and use of minimal medium in the study of bacterial genetics.

Accepted Answer

The answer of Explain the composition and use of minimal...

Question 15

Temperate phages are those that can enter either the or

Accepted Answer

Temperate phages can enter either the lytic cycle, where they destroy the host cell, or the lysogenic cycle, where they integrate their DNA into the host genome and replicate along with it without causing immediate harm.

Question 16

What is a form of recombination in bacteria that involves the F plasmid?

Accepted Answer

The answer of What is a form of recombination in...

Question 17

What are prototrophs?

Accepted Answer

The answer of What are prototrophs?...

Question 18

A bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(n) ________.

Accepted Answer

A temperate bacteriophage can enter either the lytic or lysogenic cycle, allowing it to integrate its genome into the host's DNA and remain dormant before eventually entering the lytic cycle.

Question 19

The clearing made by bacteriophages in a "lawn" of bacteria on an agar plate is called a ________.

Accepted Answer

The clearing made by bacteriophages in a lawn of bacteria on an agar plate is called a plaque. Plaques are areas where the bacteriophages have lysed the bacterial cells.

Question 20

What is the role of the F factor in bacterial recombination?

Accepted Answer

The answer of What is the role of the F...

Quiz 8: Genetic Analysis and Mapping in Bacteria and Bactierophages

In what way was the interrupted mating technique used to generate a genetic map in E. coli based on time?