In Sanger sequencing,what causes DNA synthesis to terminate at a specific base?

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In Sanger sequencing, dideoxynucleotides (ddNTPs) are used as chain terminators. These nucleotides lack a hydroxyl group at the 3' position, which is necessary for the formation of the phosphodiester bond with the next incoming nucleotide. When a ddNTP is incorporated into the growing DNA chain instead of a regular nucleotide, DNA synthesis terminates because there is no 3' hydroxyl group for the next nucleotide to attach to. This results in a set of labeled fragments that represents the DNA sequence.

In Sanger Sequencing,what Causes DNA Synthesis to Terminate at a Specific