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Genomic DNA Is Isolated from Bacteria

Question 15

Multiple Choice

Genomic DNA is isolated from bacteria.After a long digestion with a 6-base cutting enzyme that recognizes the sequence GAATTC,the DNA is not cut.The genome is 4 Mb,so there should be approximately 1,000 sites in the DNA.What is the most likely explanation?


A) There are no Gs in the genome.
B) There are no As in the genome.
C) The bacterium methylated the DNA,because it produces the 6-base cutting enzyme.
D) All the recognition sites are in genes,and the enzyme will not cut in genes.
E) All the recognition sites are in promoters and regulatory sequences,and the enzyme cuts only in genes.

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