A circular DNA of 1 kb length is cut with a restriction enzyme whose precise recognition sequence is not known.The digest yields four fragments of approximately equal size.What is the most likely conclusion from these data?

Question

Quizplus · Accepted Answer

Given that the DNA is cut into four fragments of approximately equal size, it suggests that the enzyme cuts the DNA at regular intervals. A 4-base cutter would recognize more frequent sites in a given length of DNA compared to 6-base or 8-base cutters, leading to a higher number of cuts over the same length, which aligns with the observation of four fragments from a 1 kb circular DNA.

A Circular DNA of 1 Kb Length Is Cut with a Restriction