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When Bacterial Transformants Containing a Library Are Transferred to Nitrocellulose

Question 35

Multiple Choice

When bacterial transformants containing a library are transferred to nitrocellulose filters and screened,one of the steps is treatment with an alkaline solution.What does this do?


A) attaches the library DNA to the nitrocellulose
B) promotes hybridization between the probe and complementary inserts
C) labels the DNA so it can be visualized
D) stimulates DNA replication,to increase the plasmid copy number
E) lyses the cells and denatures the released DNA

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