The Correct Order of Steps to Follow When Using Recombinant

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

A) isolate the desired gene - purify the expressed protein - grow the transformed cells into suitable quantities - insert the gene into an expression vector - transform host cells with the expression vector.
B) purify the expressed protein - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene.
C) grow the transformed cells into suitable quantities - transform host cells with the expression vector - insert the gene into an expression vector -isolate the desired gene - purify the expressed protein.
D) transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene - insert the gene into an expression vector - purify the expressed protein.
E) isolate the desired gene - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - purify the expressed protein.