If Single- Stranded Binding protein (SSB) is NOT present during DNA replication, what would you expect to see?
A) The replisome complex would not assemble on the oriC region.
B) SSB carries the helicase protein to the open region of DNA, so hydrolysis and strand separation will not occur.
C) Helicase activity is inhibited, so DNA strands cannot be separated.
D) SSB prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed.
E) The DNA cannot bend, so hydrogen bonds in the 13- mer region of oriC remain intact.

Question

Quizplus · Accepted Answer

SSB proteins bind to single-stranded DNA and prevent the reannealing of the DNA strands, ensuring that the replication machinery can access the template strands. Without SSB, the separated strands would likely reanneal, hindering replication progress.

If Single- Stranded Binding Protein (SSB) Is NOT Present During