Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension)?
A) 95° →55° →72°
B) 45° →72° →95°
C) 95° →72° →95°
D) 75° →95° →45°
E) 95° →72° →55°

Question

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension)?
A) 95°  →55° →72°
B) 45°  →72°  →95°
C) 95°  →72° →95°
D) 75°  →95°  →45°
E) 95°  →72°  →55°

Quizplus · Accepted Answer

The correct sequence for PCR temperature cycles is: denaturation at around 95°C, annealing at a lower temperature (often around 55°C, but this can vary based on the primers used), and extension at around 72°C, which is the optimal working temperature for Taq polymerase.

Which of the Following Temperature Cycles Would You Expect to See