What is the purpose of the 3'- to- 5' exonuclease activity of DNA Polymerase?
A) Removing mismatched nucleotides at the polymerase active site increases the rate of synthesis in the 5' to 3' direction.
B) Degrade excessive nucleotides on Okazaki fragments.
C) Remove mismatched nucleotides in the template strand.
D) Some polymerases do not have exonuclease activity.
E) Remove mismatched nucleotides in the newly synthesized strand.

Question

Quizplus · Accepted Answer

The 3'- to- 5' exonuclease activity of DNA Polymerase is responsible for proofreading by removing mismatched nucleotides in the newly synthesized strand, ensuring high fidelity during DNA replication.

What Is the Purpose of the 3'- To- 5' Exonuclease