Which functional groups have been altered in a ddNTP compared to a dNTP?
A) The ddNTPs have a 2' H and a 3' OH, while dNTPs have a 2' H and a 3' H.
B) The ddNTPs have a 2' OH and a 3' H, while dNTPs have a 2' H and a 3' OH.
C) The ddNTPs have a 2' H and a 3' H, while dNTPs have a 2' H and a 3' OH.
D) The ddNTPs have a 2' H and a 3' H, while dNTPs have a 2' OH and a 3' OH.
E) The ddNTPs have a 2' OH and a 3' OH, while dNTPs have a 2' H and a 3' H.

Question

Quizplus · Accepted Answer

ddNTPs (dideoxynucleotides) lack a hydroxyl group (-OH) at the 3' carbon of the sugar ring, which is present in dNTPs (deoxynucleotides). This difference prevents the addition of another nucleotide, thus terminating DNA synthesis.

Which Functional Groups Have Been Altered in a DdNTP Compared