When F+ conjugation occurs successfully, only one copy of the F plasmid single- stranded DNA is transferred. What else must minimally occur?
A) circularization plus replication of the new recipient DNA
B) circularization of the recipient's new DNA
C) rolling circle replication in the recipient cell
D) continuation of rolling circle replication in the donor cell and replication in the recipient cell
E) stopping the rolling circle replication after one copy length, circularization, and replication

Question

Quizplus · Accepted Answer

During F+ conjugation, after the single-stranded DNA of the F plasmid is transferred to the recipient cell, it must stop rolling circle replication after one copy length, then circularize and replicate to form a double-stranded circular plasmid in the recipient cell. This ensures that both cells end up with a complete copy of the F plasmid.

When F+ Conjugation Occurs Successfully, Only One Copy of the F