In generalized transduction, a phage introduces a segment of donor DNA into the recipient cell. This is followed by recombination of the donor fragment with the recipient chromosome. Which of the following must occur?
A) degradation of one of the two strands of phage genome
B) circularization of the donor fragment before recombination
C) a single crossover between the donor segment and the recipient chromosome
D) a pair (or even number) of crossovers between the donor segment and the recipient
E) replication of the donor segment before recombination

Question

Quizplus · Accepted Answer

In generalized transduction, the donor DNA is packaged into a phage and transferred to a recipient cell, where it can recombine with the recipient's chromosome. For successful integration of the donor DNA into the recipient's chromosome, a pair (or even number) of crossovers between the donor segment and the recipient chromosome is necessary. This ensures that the donor DNA is properly aligned and integrated into the recipient's genome, maintaining the integrity and continuity of the genetic material. Single crossovers, circularization, replication of the donor segment before recombination, or degradation of phage genome strands are not requirements for this process.

In Generalized Transduction, a Phage Introduces a Segment of Donor