A scientist is setting up a co- transduction experiment to determine the order of five genes in an operon. What gene should be used as the selected marker and what type of media should be used to select for it?
A) the first gene in the operon; complete media
B) a nearby essential metabolic gene downstream of the operon; complete media
C) a nearby essential metabolic gene upstream of the operon; complete media
D) a nearby essential metabolic gene upstream of the operon; minimal media supplemented with the corresponding essential metabolite
E) the first gene in the operon; minimal media supplemented with the corresponding essential metabolite

Question

Quizplus · Accepted Answer

In a co-transduction experiment, selecting for a nearby essential metabolic gene upstream of the operon on minimal media supplemented with the corresponding essential metabolite allows for the selection of bacteria that have received the transduced operon, facilitating the determination of gene order within the operon.

A Scientist Is Setting Up a Co- Transduction Experiment to Determine